目的 本实验旨在探讨不同年生鹿茸包括:二杠茸、三岔茸和再生鹿茸的蜡片、粉片(白粉片、红粉片)、血片和骨片的颜色特征与其中所含化学成分(蛋白、多糖、磷脂)含量的相关性。方法 通过国际照明委员会(CIE L*a*b*)颜色空间,检测不同年生鹿茸中5个生长部位的色度指标;采用考马斯亮蓝比色法测定蛋白质含量,苯酚硫酸法测定多糖含量,钼蓝比色法测定磷脂含量;采用皮尔逊相关性分析,将各种鹿茸颜色与其成分进行相关性分析。结果 不同年生的5种片型鹿茸在颜色和化学成分含量上有明显的差异,皮尔逊相关性分析结果表明,鹿茸颜色参数b*与3种化学成分具明显的负相关性。结论 本实验表明,基于色度学原理的颜色测定方法可有效对不同生长期的鹿茸进行区分,并且相关性分析显示颜色数字化指标在一定程度内可以反映鹿茸化学组成的不同。
Abstract
OBJECTIVE To investigate the color and chemical composition of the wax slices, w-powder slices, r-powder slices, blood slices and bone slices of two-branch Velvet Antler, three-branch Velvet Antler and reborn Velvet Antler with different growth years, and analyze the relationship between the color characteristics and chemical composition (protein, polysaccharide, phospholipid). METHODS The color parameters of five kinds of slices with different growth periods were detected by CIEL*a*b* color space. The content of protein was determined by Coomassie blue colorimetric method, the content of polysaccharide was determined by phenol sulfuric acid method, and the content of phospholipid was determined by molybdenum blue colorimetric method. Pearson correlation analysis was used to analyze the correlation between color and components. RESULTS There were significant differences in color and chemical composition between the five types of Velvet Antlers with different grow periods. Pearson correlation analysis showed that the Velvet Antler color parameter b* had a significant negative correlation with the three chemical components. CONCLUSION The color determination method based on the principle of colorimetry can effectively distinguish Velvet Antlers in different growth stages. And the correlation analysis showed that the color digital index can reflect the difference in chemical composition of Velvet Antler in a certain degree.
关键词
鹿茸 /
色度指标 /
化学成分 /
相关性
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Key words
Velvet Antler /
CIE L*a*b* /
chemical composition /
correlation
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中图分类号：
R917
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参考文献
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脚注
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基金
吉林省科技发展计划项目资助(20180414071GH)
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